Evaluation of In vitro antibacterial activity Vibrio cholera using silver nanoparticles synthesized from banana peel extract

Abstract

Recent advances in nanotechnology and the synthesis of nanoparticles
through biosynthesis have increased the urge in scientists than for
chemical or physical methods. The biosynthesis method is the most
significant method than a conventional method because of its ecofriendly,
low cost and rapid synthesizing process. The present study
describes the antibacterial activity of silver nanoparticles (AgNPs)
synthesized from banana peel extracts. The synthesis of AgNPs was
confirmed by color change from light yellow to brown color. Further,
the morphology of the biosynthesized nanoparticles, average size and
presence of functional groups were characterized by UV – Visible
spectroscopy (UV-Vis), X-Ray diffraction (XRD) and Fourier transform
infrared spectroscopy, respectively. The UV spectra results show a
strong resonance center and surface of silver nanoparticles at 450 nm.
XRD studies revealed that the synthesized AgNPs show crystalline in
shape. The FT-IR spectrum described the biological molecules which
stabilize and form the silver nanoparticles in the aqueous medium. The
antimicrobial property of AgNPs was tested against Vibrio cholerae,
which showed maximum zones of inhibition of 32 mm at a
concentration of 100 μL. Therefore, the biosynthesized AgNPs proved
to have significant antibacterial activity.