Rapid in vitro clonal propagation of two hybrid muskmelon cultivars and their field evaluation in agro climatic condition of Bangladesh
DOI:
https://doi.org/10.63799/Keywords:
Micro-propagation,, Seedling explants,, Shoot multiplication,, Adventitious rooting,, Plantlets regeneration,, Field evaluation,, Hybrid muskmelon.Abstract
An attempt was made to develop a large scale production of planting materials of two Japanese hybrid
cultivars of muskmelon through micro-propagation. Highest percentage of seed germination was found in
MS medium supplemented with 0.2 mg/l GA3. In the present work, shoot tip explant of two muskmelon
cultivars viz. Sweet Gold and Midori excised from axenic seedlings were cultured for shoot proliferation.
The maximum efficiency of shoot mul plica on was recorded in MS medium supplemented with 2.0 mg/l
BA and maximum number and length of shoots were achieved in the same concentration of BA. The
highest percentage of in vitro grown cultured shoots was rooted when they were cultured in MS rooting
medium containing 0.5 mg/l NAA for each cul var. Successfully acclimatized plantlets were transplanted
to the field and 80% plantlets survived. Field grown plantlets showed wide ranges of varia ons between
the two sources of explants for different agronomic characters such as plant length, number of
leaves/plant, number of fruits/plant and average weight/fruit.
