Assessment genetic diversity among some Egyptian bread wheat (Triticum aestivum L.) cultivars using RAPD markers

Authors

  • Khaled F. M. Salem Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt, (2) Biology Department, College of Science and Humanitarian Studies, Shaqra University, Qwaieah 11971, Saudi Arabia, Author
  • Elsayed A. Elabsawy Bioinformatics Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, P. O. Box, 79, Egypt. Author
  • Yehia A. Khidr Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt, Author
  • Hamdy Elshamy Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt Author

DOI:

https://doi.org/10.63799/

Keywords:

Bread wheat (Triticum aestivum L.),, RAPD markers,, Genetic diversity,, Polymorphism information content (PIC).

Abstract

Genetic diversity was investigated among seven bread wheat varieties originated from Egypt by 17 RAPD markers and twelve agronomic traits. The RAPD markers were capable of detecting 62 alleles with an average of 3.64 alleles per marker. The number of alleles per marker ranged from 3 to 6 and the allelic polymorphism information content (PIC) value ranged from 0.571 for the OPA-07, OPO-03 and OPO-12 to 0.816 for the OPA-01 with an average of 0.681. The results revealed that the varieties differed for RAPD markers and agronomic traits. The significant correlation coefficient between gene diversity and the number of polymorphic bands was high, r = 0.733 (P <0.01). Cluster analysis was conducted based on RAPD to group the wheat varieties and construct a dendrogram. Three main groups were distinguished by RAPD. RAPD markers showed a high level of polymorphism among the varieties examined. The present study indicates that RAPD markers could be successfully used in genetic characterization and diversity in wheat. Also, Information generated from this study can be used to select parents for hybrid development to maximize yield and its components.

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Published

2025-05-25

Issue

Vol. 9 No. 1 (2021)

Section

Research Paper

How to Cite

Assessment genetic diversity among some Egyptian bread wheat (Triticum aestivum L.) cultivars using RAPD markers. (2025). Journal of Genetic and Environment Conservation, 9(1), 168-175. https://doi.org/10.63799/