Molecular characterization of some tomato genotypes using inter simple sequence repeats (ISSR) technique
DOI:
https://doi.org/10.63799/Keywords:
Molecular characterization,, Tomato,, ISSR,, Genotypes,, Wild species.Abstract
The local tomato genotypes have many important traits comparing to the introduced hybrids. Theses genotypes are considered as valuable genetic resources that should be preserved, so in this study, the genetic variability was evaluated for 11 of the most important local genotypes comparing to 4 wild species. The ISSR technique was used with 21 primers which amplified 194 bands, including 172 polymorphic bands with a polymorphism average of 519.08%. The number of total bands per primer varied from 2 to 20 with an average of 10.777. The average of PIC and GD values were 0.4.0. and 0.5080, respectively. The cluster analysis based on genetic diversity between studied genotypes showed that the studied genotypes were divided into 5 groups, each group included one of the 4 wild species and the fifth one included all local genotypes. This study proved that the ISSR technique was succeeded in distinguishing between different tomato genotypes on molecular level.
